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"Measuring DNA quality by digital PCR using probability calculations" by David Gleerup, Yao Chen et al.

Background: Accurate methods to assess DNA integrity are needed for many biomolecular methods. A multiplex digital PCR (dPCR) method designed for interspaced target sequences can be used to assess sequence integrity of large DNA strands. The ratio of single positive partitions versus double positive partitions is then used to calculate the sheared DNA strands. However, this simple calculation is only valid with low DNA concentration. We here describe a method based on probability calculations which enables DNA quality analysis in a large dynamic range of DNA concentrations. Results: Known DNA integrity percentages were mimicked using artificial double stranded DNA in low, intermediate and high DNA concentration scenarios, respectively 600, 12500 and 30000 copies of DNA per reaction. At low concentrations both methods were similar. However, at the intermediate concentration (12500 copies per reaction) the ratio based method started producing a larger error than the proposed probability ....

Digital Pcr , Na Integrity ,

A Rs 3.5-lakh-crore industry at crossroads: What is the future of plastic in an increasingly climate conscious world?

As the world is looking actively to address the challenges of climate change and pollution, the plastic industry has found itself in quite a predicament as a necessary evil. ....

Plastics Exports , Plasticindia Foundation , Plastic Industry In India , Ag Polypack Pvt Ltd ,