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Frontiers | Emergence of Dengue 4 as Dominant Serotype During 2017 Outbreak in South India and Associated Cytokine Expression Profile

Dengue virus infection is prevalent in tropical and sub-tropical regions of the world, which is fatal if untreated symptomatically. Emergence of new genotype within serotypes led to enhanced severity. The objective of the study is to identify the molecular characteristics of the DENV circulated during 2017 outbreak in Tamil Nadu, India and, to investigate the role of inflammatory cytokines in different ‘serotypes’ and in ‘dengue severity’. A total of 135 suspected samples were tested for DENV infection using IgM, IgG and qPCR assay; where 76 samples were positive for DENV and analysed for twelve inflammatory cytokines using ELISA. Serotyping shows 14 DENV-1, 22 DENV-2, 7 DENV-3 and 33 DENV-4, where DENV-4 was predominant. Among seventy-six, forty-two isolates were successfully sequenced for C-prM region and grouped. A lineage shift was observed in DENV-4 genotype. Irrespective of serotypes, IFNγ was significantly elevated than control and in primary infection indicating its role in immune response. GM-CSF and IP-10 was significantly elevated in secondary infection and, can be used as prognostic biomarker for secondary infection. Our observation shows differential cytokine expression profile varied with each serotype indicating serotype/genotype-specific viral proteins might play a major role in dengue severity. DENV-4 as dominant serotype was reported in Tamil Nadu for the first time during an outbreak with a mixed Th1/Th17 cytokine expression profile that correlated with disease severity. We conclude it is essential to identify circulating viral genotype and their fitness by mutational analysis to correlate with disease severity and immune status, as this correlation will be helpful in diagnostics and therapeutics applications.

India , Australia , Koishi , Hokkaido , Japan , Taiwan , United-states , United-kingdom , New-delhi , Delhi , Brazil , Pune

Frontiers | Molecular Phylogenesis and Spatiotemporal Spread of SARS-CoV-2 in Southeast Asia

Frontiers | Molecular Phylogenesis and Spatiotemporal Spread of SARS-CoV-2 in Southeast Asia
frontiersin.org - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from frontiersin.org Daily Mail and Mail on Sunday newspapers.

Thailand , Australia , United-states , United-kingdom , Parida , Indonesia-general- , Indonesia , Minas-gerais , Acre , Brazil , Brunei , China

Odisha: Health experts visit village where Delta+ variant case was found | Bhubaneswar News

Experts have a mixed view on the danger that the Delta Plus variant may pose even as a three-member team of health department officials on Saturday vi

Deogarh , Orissa , India , Bhubaneswar , Odisha , Bramhadev-pattnaik , Ajay-parida , Bijay-mohapatra , Institute-of-life-sciences , Delta-plus , Life-sciences , Bhubaneswar-news

More black fungus cases at AIIMS Bhubaneswar | Bhubaneswar News

Number of mucormycosis (black fungus) patients at AIIMS Bhubaneswar has reached 40, including 12 suspected cases. A separate ward has been created in

Bhubaneswar , Orissa , India , Sachidananda-mohanty , Pradipta-kumar-parida , Kumar-parida , Bhubaneswar-news , Hubaneswar-latest-news , Hubaneswar-news-live , Hubaneswar-news-today , Oday-news-bhubaneswar

Selain Rina Gunawan, 7 Artis Ini Diketahui Meninggal Dunia karena COVID-19

Selain Rina Gunawan, 7 Artis Ini Diketahui Meninggal Dunia karena COVID-19
liputan6.com - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from liputan6.com Daily Mail and Mail on Sunday newspapers.

Jakarta , Jakarta-raya , Indonesia , Farida , Pertamina-simprug , Addition-rina-virtuous , Artist-this-note-die , Jakarta-news , Rina-virtuous , Jakarta-south , Read-also-responding

Farida Pasha Mulai Dikenal di Film "Guna-Guna Istri Muda", Inilah Perjalanan Karir Sang 'Mak Lampir'

Farida Pasha Mulai Dikenal di Film "Guna-Guna Istri Muda", Inilah Perjalanan Karir Sang 'Mak Lampir'
tribunnews.com - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from tribunnews.com Daily Mail and Mail on Sunday newspapers.

Jakarta , Jakarta-raya , Indonesia , Farida , Farida-start-known , Trip-career-the-mommy-enclosed , Jakarta-see , Mommy-enclosed , Death-farida , Blushes-interest , Ground-tomb-farida

Profil dan Biodata Farida Pasha Pemeran Mak Lampir, Ternyata Nenek dari Ify alyssa

Profil dan Biodata Farida Pasha Pemeran Mak Lampir, Ternyata Nenek dari Ify alyssa
tribunnews.com - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from tribunnews.com Daily Mail and Mail on Sunday newspapers.

Indonesia , Farida , Rina-hassim , Bio-farida-actor-mommy-enclosed , Artist-farida , Role-farida , Witchcraft-the-wife-young , Retaliation-witchcraft-the-wife-young , Shaman-necromancy , Queen-beach , இந்தோனேசியா