E) PSN1:4:4, ( F) PSN1:8:8. The direction of the arrows represents the position of the hydrogen bond between PTS and EE-PVA. We also investigated the hydro-binding of PTS and nanocarriers using 1H-NMR spectra (Figure 4B–F). We observed several characteristic protons in the PTS 1H-NMR spectra, such as aromatic protons in the regions of δ6.3–7.5 ppm, methyl protons at δ3.8, and hydroxyl protons at δ9.6 (Figure 4B). In addition, Figure 4 shows that the integrated intensity at δ9.6 ppm of PTS was observed in PSN with excipient ratios of 1:2:2 (Figure 4D) and 1:4:4 (Figure 4E); however, PSN with a 1:8:8 excipient ratio did not display an integrated signal (Figure 4F). Moreover, we observed that in the spectrum of PSN that had an excipient ratio of 1:8:8, these protons had shifted and the hydroxyl proton had disappeared (Table 2 and Figure 5). These results demonstrated that EE100/PVA effectively encapsulated PTS via intermolecular hydrogen-bonding and formed a stable nanoparticle formulation.