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Clinical improvement of DM1 patients reflected by reversal of disease-induced gene expression in blood | BMC Medicine

Myotonic dystrophy type 1 (DM1) is an incurable multisystem disease caused by a CTG-repeat expansion in the DM1 protein kinase (DMPK) gene. The OPTIMISTIC clinical trial demonstrated positive and heterogenous effects of cognitive behavioral therapy (CBT) on the capacity for activity and social participations in DM1 patients. Through a process of reverse engineering, this study aims to identify druggable molecular biomarkers associated with the clinical improvement in the OPTIMISTIC cohort. Based on full blood samples collected during OPTIMISTIC, we performed paired mRNA sequencing for 27 patients before and after the CBT intervention. Linear mixed effect models were used to identify biomarkers associated with the disease-causing CTG expansion and the mean clinical improvement across all clinical outcome measures. We identified 608 genes for which their expression was significantly associated with the CTG-repeat expansion, as well as 1176 genes significantly associated with the average ....

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Top tips for RNA-sequencing that involves degraded inputs

In this article, Roche Diagnostics elucidates upon some of their top tips and recommendations for anyone performing RNA-sequencing with degraded RNA inputs and demonstrate how to improve efficiency and performance. ....

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A system of coordinated autonomous robots for Lagrangian studies of microbes in the oceanic deep chlorophyll maximum


Science for just $15 USD.
Abstract
The deep chlorophyll maximum (DCM) layer is an ecologically important feature of the open ocean. The DCM cannot be observed using aerial or satellite remote sensing; thus, in situ observations are essential. Further, understanding the responses of microbes to the environmental processes driving their metabolism and interactions requires observing in a reference frame that moves with a plankton population drifting in ocean currents, i.e., Lagrangian. Here, we report the development and application of a system of coordinated robots for studying planktonic biological communities drifting within the ocean. The presented Lagrangian system uses three coordinated autonomous robotic platforms. The focal platform consists of an autonomous underwater vehicle (AUV) fitted with a robotic water sampler. This platform localizes and drifts within a DCM community, periodically acquiring samples while continuously monitoring the local environment. The sec ....

Ribogreen Invitrogen , Pacific Ocean , Lrauvs Aku , Rnalater Ambion Inc , University Physics , Robotics Research Springer , North Pacific Ocean , Supplementary Materials , Northeast Pacific , Ambion Inc , Wave Glider , Liquid Robotics , Teledyne Benthos , North Falmouth , Fragment Analyzer , Fragment Analyzer High Sensitivity , Robotics Research , Modern Physics , Springer Handbook , Ocean Engineering , பெஸிஃபிக் கடல் , பல்கலைக்கழகம் இயற்பியல் , ரோபாட்டிக்ஸ் ஆராய்ச்சி ஸ்பிரிங்கர் , வடக்கு பெஸிஃபிக் கடல் , துணை பொருட்கள் , வடகிழக்கு பெஸிஃபிக் ,