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Purpose: The deposition of hydroxyapatite (HAp) crystals plays an important role in the development of vascular calcification (VC). This study aimed to demonstrate the effects of nanosized HAp (nHAp) on vascular smooth muscle cells (VSMCs) and VC progression. Methods: Transmission electron microscopy (TEM) was used to examine cellular uptake of nHAp. Cell viability was determined using CCK-8 assay kit. Mitochondrial impairment and reactive oxygen species were detected by TEM and fluorescence dye staining, respectively. Cell apoptosis was detected by Western blot analysis and Annexin V stainin...

CircRNA-TBC1D4, circRNA-NAALAD2 and circRNA-TGFBR3: selected key circRNAs in neuroblastoma and their associations with clinical features

15 Basal epithelial cells are a blood–prostate barrier between luminal cells and basement membrane 16 but are absent in prostate carcinoma. 17–19 Loss of basal epithelial cells is a characteristic of prostate carcinoma 20,21 and one of the diagnostic criteria of prostate cancer, 19 suggesting that some essential connections might exist between basal epithelial cells and prostate cancer cells. So, we put out a question regarding whether basal epithelial cells could suppress migration or invasion of prostate cancer cells when basal epithelial cells are artificially added into a cultured sys...

Knocking Down KCNQ1OT1 Suppressed LPS-Induced Neuroinflammation and Neuronal Apoptosis in HMC3 Cells For further exploring the function of KCNQ1OT1, we transfected HMC3 cells with pc-KCNQ1OT1 or si-KCNQ1OT1. The expression of KCNQ1OT1 was evaluated by RT-qPCR. Compared with control cells (cells transfected with pc-NC or si-NC), the expression of KCNQ1OT1 in HMC3 cells transfected with pc-KCNQ1OT1 was significantly increased, while the expression of KCNQ1OT1 in cells transfected with si-KCNQ1OT1 was significantly decreased (Figure 2A). Subsequently, we used RT-qPCR and ELISA kits to examine th...

9 Aberrantly expressed CCDC144NL-AS1 was observed in gastric cancer, 9 however, its roles in HCC development and the potential mechanism have not been reported yet. In this study, we investigated the expression pattern of CCDC144NL-AS1 using human HCC tissues, and explored the underlying mechanism from the epigenetic pathway. We also estimated the therapy value of CCDC144NL-AS1 using HCC mouse model. Our study may provide novel therapeutic targets for HCC treatment to improve the prognosis of HCC. Patients and Methods Tissue Collection From April 2014 to December 2015, 135 pairs of HCC tissue...