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What We Know About SARS-CoV-2 One Year Later: Part 2


What We Know About SARS-CoV-2 One Year Later: Part 2
Sponsored Content by ACROBiosystemsApr 14 2021
The S protein of SARS-CoV had been studied extensively before the SARS-CoV-2 outbreak. Among other structural proteins of SARS-CoV-2, the S protein was discovered to be highly immunogenic and effective at neutralizing antibodies recovered from SARS patients were directed at S protein.
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Research into therapeutics and vaccines against SARS-CoV-2 was focused immediately on the S protein. Although the four structural proteins of the novel SARS-CoV share over 90% in sequence identity with SARS-CoV-2, S protein consisting of S1 and S2 subunits diverged had just 77% sequence similarity.
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The S1 subunit contains the NTD or the N-terminal domain and Receptor binding domain (RBD), which binds to the human angiotensin-converting enzyme-2 (ACE2) mainly through direct interaction with the receptor-binding motif (RBM). ....

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Brazilian SARS-CoV-2 P.1 variant shows resistance to antibody neutralization


Brazilian SARS-CoV-2 P.1 variant shows resistance to antibody neutralization
Researchers in the United States say the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variant – P.1 – that has recently emerged in Brazil shows a worrying level of resistance to neutralization by antibodies.
The SARS-CoV-2 virus is the agent responsible for the coronavirus disease 2019 (COVID-19) pandemic that poses an ongoing threat to mankind and has now claimed the lives of more than 2.56 million people globally.
The team from Columbia University in New York found that as well as being resistant to several neutralizing monoclonal antibodies (mAbs), the P.1 variant is also over six times more resistant to neutralization by convalescent plasma and more than twice as resistant to sera from vaccinees than the wildtype virus. ....

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Prolonged evolution of the human B cell response to SARS-CoV-2 infection


+CD27
+ B cells at each sampling time point (top). Mean frequencies of SARS-CoV-2 S-specific B cells expressing the indicated isotype in mild and severe donors (bottom). Error bars denote standard deviation and black bars indicate means. (F) Proportion of SARS-CoV-2 S-specific swIg
+ B cells that express CD71 at each sampling time point (top). Mean frequencies of SARS-CoV-2 S-specific swIg
+CD71
+ B cells in mild and severe donors (bottom). Error bars denote standard deviation and black bars indicate means. The dotted line indicates the level of CD71 expression on swIg
+ B cells in pre-pandemic donor samples. (G) Representative gating for SARS-CoV-2 S-specific B cells. The gated populations were single cell sorted for mAb cloning. Statistical comparisons between naive and convalescent donors were made by two-sided one-way Welsh ANOVA for unpaired samples with Dunnett’s T3 multiple comparisons test. Statistical comparisons between timepoints among convalescent ....

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Skepticism Overshadows Chinese Vaccine's Rollout in Hungary


The Chinese Sinopharm vaccine has arrived to Hungary’s GP offices, and has now effectively become a working part of the nation’s vaccination process. Prime Minister Viktor Orbán’s emphasis on urgency and the vaccine’s sudden authorization have, however, caused a growing amount of skepticism due to the lack of documentation available regarding its effacy and safety.
Chief Medical Officer Cecília Müller announced today that the Sinopharm vaccine has arrived to clinics around Hungary, reports MTI.
She emphasized that there are currently enough doses for every family doctor to inoculate an average of 50-55 people, but this is dependent on the amount of people under the GP’s treatment. Some may even receive enough for the vaccination of 70 people. ....

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