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Frontiers | Significant Difference of Immune Cell Fractions and Their Correlations With Differential Expression Genes in Parkinson's Disease

Parkinson’s disease (PD) is the second most neurodegenerative disease in the world. T cell infiltration in the central nervous system (CNS) has provided insights that the peripheral immune cells participate in the pathogenesis of PD. However, the association between the peripheral immune system and CNS is remained to be elucidated. Here, we analyzed an incorporative substantial nigra expression data as well as another blood expression data using the CIBERSORT to obtain the 22 immune cells fraction and then explored the molecular function to identify the potential key immune cell types and genes of PD. We observed the proportions of naïve CD4 T cells, gamma delta T cells, resting NK cells, neutrophils in the blood, and regulatory T cells (Tregs) in the substantial nigra were significantly different between PD patients and healthy controls (HC). We identified p53-induced death domain protein 1 (PIDD1) as the hub gene of a Parkinson’s disease-related module. The set of neuron-spe ....

United States , J Neuroimmune Pharmacol , Felix Garza , Tregs Rosenkranz , Acta Neuropathol , Guangdong Province Key Laboratory Of Fermentation , National Natural Science Foundation Of China , Graphpad Software , Differential Gene Expression , Supplementary Table , Supplementary Figures , Enrichment Analysis , Kyoto Encyclopedia , Graphpad Prism , Supplementary Figure , National Natural Science Foundation , High Level Hospital Construction Project , Guangdong Province Key Laboratory , Enzyme Engineering , Supplementary Material , Med Abstract , Crossref Full Text , Meyer Zu Horste , Parkinson 39s Disease , Immune Cells , Substantia Nigra ,

[Full text] Sinomenine Inhibits the Growth of Ovarian Cancer Cells Through the Sup


CDK1,
BUB1) play important roles in the progression of G2/M phase in the cell cycle.
30,31 Furthermore, real-time PCR analysis was used to verify the results of transcriptome sequencing and the results indicated that the expression trends of the top 18 ranked degree genes were consistent with those obtained by RNA-seq, suggesting that the RNA-seq data reliably reflected the gene expression alterations (Figure 4B). According to the RNA-seq and real-time PCR results, the expressions of cell cycle regulated genes, such as
CDK1,
PLK1, and
BUB1, were significantly decreased after sinomenine treatment. These results were also in accord with the cell phenotype experiments. ....

United States , San Diego , Dnasei Takara , Bioanalyser Agilent , Beckman Coulter Cyto , Transcriptome Library Construction , Proteintech Group , Network Analysis , Chinese Academy Of Sciences , Cell Signaling Technology , Stem Cell Bank , Shanghai Majorbio Bio Pharm Biotechnology Co Ltd , Cell Culture , Chinese Academy , Survival Rate Assay , Cell Proliferation , Yuxiu Biotech , Seq Transcriptome Library Construction , Illumina Hiseq , Thermo Scientific , Nanodrop Technologies , Low Range Ultra Agarose , Expression Analysis , Transcripts Per Kilobase , Digital Gene Expression , Bonferroni Correctedp Value ,